| Primary Identifier | MGI:2182454 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ube3a |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | 3.2 kb of the gene including part of exon 15 and all of exon 16 was replaced with a IRES-lacZ-neo cassette via homologous recombination resulting in deletion of the C-terminal region encoding ubiquitin protein ligase activity. Absence of gene expression in animals carrying a mutant maternal allele was confirmed by Western blot analysis. Beta-gal staining of brain sections from maternal-deficient animals showed high levels of lacZ activity in the hippocampus, dentate gyrus, ventricular ependyma, and in scattered cells throughout the frontal cortex. |
