| First Author | Kanayama M | Year | 2018 |
| Journal | Prostate Int | Volume | 6 |
| Issue | 3 | Pages | 99-103 |
| PubMed ID | 30140659 | Mgi Jnum | J:304912 |
| Mgi Id | MGI:6511430 | Doi | 10.1016/j.prnil.2018.04.003 |
| Citation | Kanayama M, et al. (2018) Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase. Prostate Int 6(3):99-103 |
| abstractText | Background: Genetically engineered mouse models are useful tools to decipher molecular mechanisms of diseases. As for prostates, a rat probasin promoter has been widely used to drive prostate-specific gene expression. To optimize its codon usage to that of mammals, we used codon-improved Cre recombinase (iCre) for prostate-specific Cre-loxP recombination. Materials and methods: We generated transgenic mice that express iCre driven by conventional probasin promoter in a prostate-specific manner (PB-iCre). Linearized PB-iCre transgene deoxyribonucleic acids (DNAs) were microinjected into pronuclei of fertilized mouse embryos. The integration of the transgene was confirmed by Southern blot analysis. A line of transgenic mice expressing a sufficient amount of iCre mRNA in its prostate was selected. To test recombinase activity of PB-iCre in vivo, its offspring was crossbred with Pten(flox/flox) mice in which murine prostate adenocarcinoma is reported to occur upon excision of loxP-flanked regions. Results: Eight founder animals were obtained, all of which showed germ line integration of PB-iCre transgene by Southern blot analysis. Among them, the prostate from only one line (line 58) expressed a sufficient amount of iCre mRNA. This line was crossbred with Pten(flox/flox) mice to generate PB-iCre58/Pten(flox/flox). As a result, 12-week-old PB-iCre58/Pten(flox/flox) mice presented with prostate adenocarcinoma that was histologically similar to human cribriform prostate cancer of Gleason grade 4. Conclusions: We have successfully established a transgenic mouse line that expresses iCre in a prostate-specific manner. |
