| First Author | Fu Y | Year | 2019 |
| Journal | Biomed Res Int | Volume | 2019 |
| Pages | 3027036 | PubMed ID | 30809535 |
| Mgi Jnum | J:294821 | Mgi Id | MGI:6445380 |
| Doi | 10.1155/2019/3027036 | Citation | Fu Y, et al. (2019) Vitamin D Regulates the Expressions of AQP-1 and AQP-4 in Mice Kidneys. Biomed Res Int 2019:3027036 |
| abstractText | Aim: Vitamin D plays an important role in water and salt homeostasis. The aim of our study was to investigate the underlying relationship of Vitamin D and Aquaporins (AQP). Methods: The behaviors of 1alpha (OH)-ase knockout mice and wild type mice were observed before analysis. The ICR mice were treated with vehicle or paricalcitol, a vitamin D analogue, followed by animals receiving a standard diet and free access to drinking water either with aliskiren (renin blocker; 37.5 mg aliskiren in 100 ml water), or telmisartan (a angiotensin II type I receptor blocker; 40 mg telmisartan in 100 ml water) a week before study. The expressions of AQP-1, AQP-4, and renin in mice kidneys were detected by western bolting, immunohistochemistry, and immunofluorescence. Results: Diuresis and polydipsia were observed in 1alpha (OH)-ase knockout mice, and a decreased water intake and urine output in ICR mice was observed after paricalcitol treatment. Compared with wild type, the AQP-1 expressions were increased in renal papilla and AQP-4 expressions were decreased in renal proximal tubule of 1alpha(OH) ase knockout mice. In addition, AQP-1 was decreased in renal papilla and AQP-4 expressions were increased in proximal tubule by suppressing renin activity or supplement of Vitamin D analogue. After injecting renin into the lateral ventricle of the 1alpha(OH)ase knockout mice, the renin expression level was decreased in the kidney, followed by the decrease of AQP-1 in renal papilla and increase of AQP-4 in proximal tubule. Conclusions: Overall, Vitamin D and renin inhibitors have synergistic effects in regulating water channels in mice kidneys. |
