| First Author | Griener A | Year | 2015 |
| Journal | Dev Neurobiol | Volume | 75 |
| Issue | 11 | Pages | 1189-203 |
| PubMed ID | 25649879 | Mgi Jnum | J:242402 |
| Mgi Id | MGI:5905127 | Doi | 10.1002/dneu.22277 |
| Citation | Griener A, et al. (2015) Probing diversity within subpopulations of locomotor-related V0 interneurons. Dev Neurobiol 75(11):1189-203 |
| abstractText | The V0 interneuronal population is derived from Dbx1 expressing progenitors. Initial studies on these interneurons in the mouse spinal cord demonstrated that they project commissural axons and are involved in coordinating left-right alternation during locomotion. Subsequent work has indicated that the V0 population can be divided into genetically distinct ventral (V0V) and dorsal (V0D) subpopulations, and experimental evidence suggests that each is responsible for left-right alternation at different locomotor speeds. In this study, we perform a series of experiments to probe the location and connectivity of these subpopulations in neonatal mice and demonstrate that they are more diverse than previously predicted. While the distribution of either subpopulation remains consistent along the extent of the lumbar spinal cord, a cluster of V0D cells lateral to the central canal receive substantial input from primary afferents. Retrograde tracing and activity dependent labeling experiments demonstrate that a group of V0 interneurons located in this same region preferentially project axons towards contralateral motoneurons via an oligosynaptic pathway, and are active during fictive locomotion. Our results suggest that this subset of V0 interneurons may be primarily responsible for coordination of left-right alternation during locomotion. Furthermore these experiments indicate that while genetic identity is one determinant of the function of a neuron during locomotion, the specific position in which the cell is located may also play a key role. |
