| First Author | Rosen ED | Year | 2005 |
| Journal | Thromb Haemost | Volume | 94 |
| Issue | 3 | Pages | 493-7 |
| PubMed ID | 16268461 | Mgi Jnum | J:144540 |
| Mgi Id | MGI:3831177 | Citation | Rosen ED, et al. (2005) Generation of genetically-altered mice producing very low levels of coagulation factorVII. Thromb Haemost 94(3):493-7 |
| abstractText | It has been shown earlier that mice with a total targeted deletion of the factorVII gene (FVII(-/-)) die perinatally, thereby precluding study of adult animals with this total deficiency. Consequently, mice producing very low levels of FVII were developed by targeted replacement of the wild-type (WT) murine FYII gene with its corresponding cDNA, under control of the tetracycline transactivator (tTA) promoter. When backcrossed into the C57BI/6 strain, unchallenged mice containing two replaced FVII(tTA) alleles (FVII(tTA/tTA) produce approximately 0.7% of WT FVII levels, but yet live to adulthood despite displaying severely downregulated overall thrombin production and spontaneously developing cardiac fibrosis at a young adult age.This genetically-altered mouse line provides an excellent animal model to study consequences of a severe FVII deficiency in unchallenged mice and in mice subjected to a variety of experimental challenges. |
