| First Author | Clements JL | Year | 2006 |
| Journal | J Immunol | Volume | 177 |
| Issue | 2 | Pages | 905-12 |
| PubMed ID | 16818745 | Mgi Jnum | J:125056 |
| Mgi Id | MGI:3723406 | Doi | 10.4049/jimmunol.177.2.905 |
| Citation | Clements JL, et al. (2006) Impaired generation of CD8+ thymocytes in Ets-1-deficient mice. J Immunol 177(2):905-12 |
| abstractText | The Ets family of transcription factors function as key regulators of multiple aspects of immune cell development and function. To date, Ets-1 has been implicated in regulating early stages of thymic maturation and lymphocyte function and homeostasis. This report describes a novel role for Ets-1 in supporting later stages of thymic selection, in that positive selection of MHC class I-restricted CD4+CD8+ double-positive thymocytes is markedly inhibited in mice expressing a hypomorphic allele of Ets-1. This effect is thymocyte intrinsic, as Ets-1 mutant thymocytes fail to efficiently generate CD8+ single-positive thymocytes in mixed bone marrow chimeric backgrounds. Although peripheral CD8+ T cells are present in Ets-1 mutant mice, both CD4+ and CD8+ subsets contain an elevated proportion of cells with an effector memory (CD62L-CD44+) phenotype. In addition, while thymic expression of Thy1 is relatively normal, peripheral T cells isolated from Ets-1 mutant mice display a striking loss of Thy1 expression. These data identify Ets-1 as a key transcription factor regulating thymocyte positive selection and lineage commitment of MHC class I-restricted thymocytes. |
