| Primary Identifier | MGI:2182620 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Hsf2 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene is disrupted by insertion of a lacZ-neo cassette into exon 5 via homologous recombination. The gene targeting event results in the disruption of the oligomerization domain at lysine 167 and disruption of one of two nuclear localization signals while HSE-binding activity is retained. The mutant protein is expected to be cytoplasmic and lack trimerization abilities. RT-PCR and Western blot analyses confirmed the absence of gene expression in testes of homozygous mutant animals and homozygous mutant embryos. |
