| First Author | Favre J | Year | 2023 |
| Journal | Int J Mol Sci | Volume | 24 |
| Issue | 20 | PubMed ID | 37894719 |
| Mgi Jnum | J:358073 | Mgi Id | MGI:7546228 |
| Doi | 10.3390/ijms242015038 | Citation | Favre J, et al. (2023) NTPDase1/CD39 Ectonucleotidase Is Necessary for Normal Arterial Diameter Adaptation to Flow. Int J Mol Sci 24(20) |
| abstractText | NTPDase1/CD39, the major vascular ectonucleotidase, exerts thrombo-immunoregulatory function by controlling endothelial P2 receptor activation. Despite the well-described release of ATP from endothelial cells, few data are available regarding the potential role of CD39 as a regulator of arterial diameter. We thus investigated the contribution of CD39 in short-term diameter adaptation and long-term arterial remodeling in response to flow using Entpd1(-/-) male mice. Compared to wild-type littermates, endothelial-dependent relaxation was modified in Entpd1(-/-) mice. Specifically, the vasorelaxation in response to ATP was potentiated in both conductance (aorta) and small resistance (mesenteric and coronary) arteries. By contrast, the relaxing responses to acetylcholine were supra-normalized in thoracic aortas while decreased in resistance arteries from Entpd1(-/-) mice. Acute flow-mediated dilation, measured via pressure myography, was dramatically diminished and outward remodeling induced by in vivo chronic increased shear stress was altered in the mesenteric resistance arteries isolated from Entpd1(-/-) mice compared to wild-types. Finally, changes in vascular reactivity in Entpd1(-/-) mice were also evidenced by a decrease in the coronary output measured in isolated perfused hearts compared to the wild-type mice. Our results highlight a key regulatory role for purinergic signaling and CD39 in endothelium-dependent short- and long-term arterial diameter adaptation to increased flow. |
