| Primary Identifier | MGI:2183211 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rasgrf2 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A portion of the CDC25-H domain, a highly conserved region of the gene, from amino acids 1005-1070 was replaced with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of brain RNA and absence of protein product was demonstrated by Western blot of brain and lung extracts using an antibody directed against a region of the protein upstream of the disruption. |
