| Primary Identifier | MGI:2182645 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nbn |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 2 and 3 encoding amino acids 12-194 were replaced with a PGK-neo cassette via homologous recombination. RT-PCR analysis of mouse embryonic fibroblasts (MEFs) from homozygous mutant animals using primers 5' of exon 2 and 3' of exon 3 detected low levels of truncated mRNA resulting from the splicing of exon 1 to exon 4, and sequencing of the product confirmed the deletion of exons 2 and 3. Western blot analysis of MEFs from homozygous mutants detected low levels of truncated protein product. |
