| Primary Identifier | MGI:2182685 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gne |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was replaced with a neomycin resistance gene cassette via homologous recombination. Homozygous mutant embryos were observed at a frequency of 10% and 6% at E8.5 and E9.5, respectively, by PCR genotype analysis. Because homozygous mutant animals could not be obtained, embryonic stem cell lines from E3.5 homozygous mutant embryos were established. RT-CR and Western blot analysis of the mutant stem cell lines confirmed the absence of gene expression. |
