| First Author | Kim JB | Year | 2007 |
| Journal | Science | Volume | 316 |
| Issue | 5830 | Pages | 1481-4 |
| PubMed ID | 17556586 | Mgi Jnum | J:122171 |
| Mgi Id | MGI:3713434 | Doi | 10.1126/science.1137325 |
| Citation | Kim JB, et al. (2007) Polony multiplex analysis of gene expression (PMAGE) in mouse hypertrophic cardiomyopathy. Science 316(5830):1481-4 |
| abstractText | We describe a sensitive mRNA profiling technology, PMAGE (for 'polony multiplex analysis of gene expression'), which detects messenger RNAs (mRNAs) as rare as one transcript per three cells. PMAGE incorporates an improved ligation-based method to sequence 14-nucleotide tags derived from individual mRNA molecules. One sequence tag from each mRNA molecule is amplified onto a separate 1-micrometer bead, denoted as a polymerase colony or polony, and about 5 million polonies are arrayed in a flow cell for parallel sequencing. Using PMAGE, we identified early transcriptional changes that preceded pathological manifestations of hypertrophic cardiomyopathy in mice carrying a disease-causing mutation. PMAGE provided a comprehensive profile of cardiac mRNAs, including low-abundance mRNAs encoding signaling molecules and transcription factors that are likely to participate in disease pathogenesis. |
