| First Author | Cowan JE | Year | 2018 |
| Journal | Eur J Immunol | Volume | 48 |
| Issue | 5 | Pages | 844-854 |
| PubMed ID | 29285761 | Mgi Jnum | J:262956 |
| Mgi Id | MGI:6160651 | Doi | 10.1002/eji.201747375 |
| Citation | Cowan JE, et al. (2018) Aire controls the recirculation of murine Foxp3(+) regulatory T-cells back to the thymus. Eur J Immunol 48(5):844-854 |
| abstractText | In the thymus, medullary thymic epithelial cells (mTEC) determine the fate of newly selected CD4(+) and CD8(+) single positive (SP) thymocytes. For example, mTEC expression of Aire controls intrathymic self-antigen availability for negative selection. Interestingly, alterations in both Foxp3(+) Regulatory T-cells (T-Reg) and conventional SP thymocytes in Aire(-/-) mice suggest additional, yet poorly understood, roles for Aire during intrathymic T-cell development. To examine this, we analysed thymocytes from Aire(-/-) mice using Rag2GFP and Foxp3 expression, and a recently described CD69/MHCI subset definition of post-selection CD4(+) conventional thymocytes. We show that while Aire is dispensable for de novo generation of conventional alphabetaT-cells, it plays a key role in controlling the intrathymic T-Reg pool. Surprisingly, a decline in intrathymic T-Reg in Aire(-/-) mice maps to a reduction in mature recirculating Rag2GFP(-) T-Reg that express CCR6 and re-enter the thymus from the periphery. Furthermore, we show mTEC expression of the CCR6 ligand CCL20 is reduced in Aire(-/-) mice, and that CCR6 is required for T-Reg recirculation back to the thymus. Collectively, our study re-defines requirements for late stage intrathymic alphabetaT-cell development, and demonstrates that Aire controls a CCR6-CCL20 axis that determines the developmental makeup of the intrathymic T-Reg pool. |
