| Primary Identifier | MGI:2183170 | Allele Type | Targeted |
| Gene | Gabra5 | Transmission | Germline |
| Strain of Origin | 129X1/SvJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | This allele was generated by breeding mice carrying Gabra5tm1Uru with transgenic mice expressing cre recombinase via the EIIa promoter. The floxed neomycin cassette was excised from intron 3, while the point mutation in codon 105 that results in a histidine to arginine substitution remained intact. Western blot analysis showed that the expression of the encoded protein was decreased by approximately 20% in homozygous mutant mice. Autoradiographs showed novel diazepam-insensitve sites in regions where the endogenous protein is expressed, indicating the disruption of the drug-binding site. |
