| First Author | Makara JK | Year | 2003 |
| Journal | Mol Cell Neurosci | Volume | 23 |
| Issue | 4 | Pages | 521-30 |
| PubMed ID | 12932434 | Mgi Jnum | J:85385 |
| Mgi Id | MGI:2674210 | Doi | 10.1016/s1044-7431(03)00080-0 |
| Citation | Makara JK, et al. (2003) Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury. Mol Cell Neurosci 23(4):521-30 |
| abstractText | Chloride channels are important for astrocytic volume regulation and K+ buffering. We demonstrate functional expression of a hyperpolarization-activated Cl- current in a subpopulation of astrocytes in acute slices or after fresh isolation from adult brain of GFAP/EGFP transgenic animals in which astrocytes are selectively labeled. When Na+ and K+ were substituted with NMDG+ and Cs+ in extra- and intracellular solutions, an inward current was observed at negative membrane potentials. The current displayed features as described for a Cl- current characterized in cultured astrocytes: it activated time dependently at potentials negative to -40 mV, displayed no inactivation within 1 s, and was inhibited reversibly by submicromolar concentrations of Cd2+. The current was not detectable in astrocytes from ClC-2 knockout mice, indicating that the ClC-2 chloride channel generated the conductance. Current density was significantly lower in a corresponding population of astrocytes isolated from immature brain and in reactive astrocytes within a lesion site. |
