| Primary Identifier | MGI:2183297 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Clcn3 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3, encoding the first transmembrane domain, and surrounding intronic sequence was replaced with a neomycin selection cassette inserted by homologous recombination. The absence of transcript in homozygous mutant mice was determined by an RNAse protection assay of brain RNA. Western blot analysis, using a specific antibody directed against the amino terminal region, indicated a lack of encoded protein. |
