| Primary Identifier | MGI:2383980 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cd55 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was introduced with a neo-TK cassette into exon 3 and a second loxP site was introduced with a TK-Hph cassette into exon 5 via homologous recombination. Transient expression of cre recombinase in correctly targeted ES cells removed floxed regions containing exons 3-5 and the 2 TK genes. The deleted exons encode complement control protein (CCP) modules 2 and 3. RT-PCR analysis detected a truncated mRNA in homozygous mutant animals lacking exons 3-5. Functional assays confirmed the mutant protein was devoid of protein activity. |
