| First Author | Voets T | Year | 2001 |
| Journal | Neuron | Volume | 31 |
| Issue | 4 | Pages | 581-91 |
| PubMed ID | 11545717 | Mgi Jnum | J:107685 |
| Mgi Id | MGI:3621672 | Doi | 10.1016/s0896-6273(01)00391-9 |
| Citation | Voets T, et al. (2001) Munc18-1 promotes large dense-core vesicle docking. Neuron 31(4):581-91 |
| abstractText | Secretory vesicles dock at the plasma membrane before Ca(2+) triggers their exocytosis. Exocytosis requires the assembly of SNARE complexes formed by the vesicle protein Synaptobrevin and the membrane proteins Syntaxin-1 and SNAP-25. We analyzed the role of Munc18-1, a cytosolic binding partner of Syntaxin-1, in large dense-core vesicle (LDCV) secretion. Calcium-dependent LDCV exocytosis was reduced 10-fold in mouse chromaffin cells lacking Munc18-1, but the kinetic properties of the remaining release, including single fusion events, were not different from controls. Concomitantly, mutant cells displayed a 10-fold reduction in morphologically docked LDCVs. Moreover, acute overexpression of Munc18-1 in bovine chromaffin cells increased the amount of releasable vesicles and accelerated vesicle supply. We conclude that Munc18-1 functions upstream of SNARE complex formation and promotes LDCV docking. |
