| First Author | Kwon YT | Year | 2002 |
| Journal | Science | Volume | 297 |
| Issue | 5578 | Pages | 96-9 |
| PubMed ID | 12098698 | Mgi Jnum | J:77750 |
| Mgi Id | MGI:2182515 | Doi | 10.1126/science.1069531 |
| Citation | Kwon YT, et al. (2002) An essential role of N-terminal arginylation in cardiovascular development. Science 297(5578):96-9 |
| abstractText | The enzymatic conjugation of arginine to the N-termini of proteins is a part of the ubiquitin-dependent N-end rule pathway of protein degradation. In mammals, three N-terminal residues-aspartate, glutamate, and cysteine-are substrates for arginylation. The mouse ATE1 gene encodes a family of Arg-tRNA-protein transferases (R-transferases) that mediate N-terminal arginylation. We constructed ATE1-lacking mouse strains and found that ATE1-/- embryos die with defects in heart development and in angiogenic remodeling of the early vascular plexus. Through biochemical analyses, we show that N-terminal cysteine, in contrast to N-terminal aspartate and glutamate, is oxidized before its arginylation by R-transferase, suggesting that the arginylation branch of the N-end rule pathway functions as an oxygen sensor. |
