| First Author | Schuler M | Year | 2005 |
| Journal | Genesis | Volume | 41 |
| Issue | 4 | Pages | 165-70 |
| PubMed ID | 15789425 | Mgi Jnum | J:97313 |
| Mgi Id | MGI:3575214 | Doi | 10.1002/gene.20107 |
| Citation | Schuler M, et al. (2005) Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse. Genesis 41(4):165-70 |
| abstractText | To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA-Cre-ER(T2) mouse line in which the expression of the tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the human skeletal muscle alpha-actin gene, contained in a P1-derived artificial chromosome. In this transgenic line Cre-ER(T2) is selectively expressed in skeletal muscles, and Cre-ER(T2)-mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle-specific and strictly tamoxifen-dependent. HSA-Cre-ER(T2) mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders. genesis 41:165-170, 2005. (c) 2005 Wiley-Liss, Inc. |
