| Primary Identifier | MGI:2183818 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Lefty2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 0.5 kb region of the gene containing the asymmetric enhancer (ASE) was replaced with a loxP site flanked neomycin resistance gene cassette via homologous recombination. The floxed neo cassette was subsequently removed by crossing chimeric animals to a cre-expressing line. Ablation of asymmetric gene expression in homozygous mutant embryos was confirmed by whole mount in situ hybridization. |
