| First Author | Cosway EJ | Year | 2017 |
| Journal | J Exp Med | Volume | 214 |
| Issue | 11 | Pages | 3183-3195 |
| PubMed ID | 28830910 | Mgi Jnum | J:249596 |
| Mgi Id | MGI:5922507 | Doi | 10.1084/jem.20171000 |
| Citation | Cosway EJ, et al. (2017) Redefining thymus medulla specialization for central tolerance. J Exp Med 214(11):3183-3195 |
| abstractText | During alphabetaT cell development, the thymus medulla represents an essential microenvironment for T cell tolerance. This functional specialization is attributed to its typical organized topology consisting of a branching structure that contains medullary thymic epithelial cell (mTEC) networks to support negative selection and Foxp3(+) T-regulatory cell (T-reg) development. Here, by performing TEC-specific deletion of the thymus medulla regulator lymphotoxin beta receptor (LTbetaR), we show that thymic tolerance mechanisms operate independently of LTbetaR-mediated mTEC development and organization. Consistent with this, mTECs continue to express Fezf2 and Aire, regulators of intrathymic self-antigens, and support T-reg development despite loss of LTbetaR-mediated medulla organogenesis. Moreover, we demonstrate that LTbetaR controls thymic tolerance by regulating the frequency and makeup of intrathymic dendritic cells (DCs) required for effective thymocyte negative selection. In all, our study demonstrates that thymus medulla specialization for thymic tolerance segregates from medulla organogenesis and instead involves LTbetaR-mediated regulation of the thymic DC pool. |
