| First Author | Kraus M | Year | 1999 |
| Journal | Immunity | Volume | 11 |
| Issue | 5 | Pages | 537-45 |
| PubMed ID | 10591179 | Mgi Jnum | J:279159 |
| Mgi Id | MGI:6359358 | Doi | 10.1016/s1074-7613(00)80129-6 |
| Citation | Kraus M, et al. (1999) Ig-alpha cytoplasmic truncation renders immature B cells more sensitive to antigen contact. Immunity 11(5):537-45 |
| abstractText | To study the function of Ig-alpha in the selection of autoreactive B cells, we have analyzed mb-1 cytoplasmic truncation mutant mice (mb-1delta(c)/delta(c)), which coexpress transgenes encoding hen egg lysozyme (HEL) and HEL-specific immunoglobulin. We demonstrate that in the presence of soluble HEL (sHEL) and dependent on the mb-1delta(c) mutation, most immature B cells bearing the HEL-specific Ig transgene undergo rearrangements of endogenous kappa light chains, resulting in loss of HEL specificity. Moreover, immature B cells from Ig-alpha mutant mice respond to BCR cross-linking with an exaggerated and prolonged calcium response and induction of protein tyrosine phosphorylation. Our data imply a negative signaling role for Ig-alpha in immature B cells. |
