| First Author | Zhang Y | Year | 2024 |
| Journal | Cell | Volume | 187 |
| Issue | 21 | Pages | 6104-6122.e25 |
| PubMed ID | 39276776 | Mgi Jnum | J:358178 |
| Mgi Id | MGI:7779298 | Doi | 10.1016/j.cell.2024.08.026 |
| Citation | Zhang Y, et al. (2024) Long-term mesoscale imaging of 3D intercellular dynamics across a mammalian organ. Cell 187(21):6104-6122.e25 |
| abstractText | A comprehensive understanding of physio-pathological processes necessitates non-invasive intravital three-dimensional (3D) imaging over varying spatial and temporal scales. However, huge data throughput, optical heterogeneity, surface irregularity, and phototoxicity pose great challenges, leading to an inevitable trade-off between volume size, resolution, speed, sample health, and system complexity. Here, we introduce a compact real-time, ultra-large-scale, high-resolution 3D mesoscope (RUSH3D), achieving uniform resolutions of 2.6 x 2.6 x 6 mum(3) across a volume of 8,000 x 6,000 x 400 mum(3) at 20 Hz with low phototoxicity. Through the integration of multiple computational imaging techniques, RUSH3D facilitates a 13-fold improvement in data throughput and an orders-of-magnitude reduction in system size and cost. With these advantages, we observed premovement neural activity and cross-day visual representational drift across the mouse cortex, the formation and progression of multiple germinal centers in mouse inguinal lymph nodes, and heterogeneous immune responses following traumatic brain injury-all at single-cell resolution, opening up a horizon for intravital mesoscale study of large-scale intercellular interactions at the organ level. |
