| Primary Identifier | MGI:2384355 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(RP23-291P1)2Flp |
| Strain of Origin | 129S1/SvImJ | Is Recombinase | false |
| Is Wild Type | false |
| description | The primary publication does not distinguish, in describing experimental results, between this and another transgenic line, designated Tg(RP23-291P1)1Flp, generated in the same strain (129S1/SvImJ) using this BAC. Some differences, though not statistically significant, were noted in semi-quantitative scores of gallbladder biles of transgenic vs. wild-type 129S1/SvImJ mice fed a lithogenic diet for 4 weeks for:
Densitometric analysis of northern blots of total liver RNA from chow-fed mice revealed Cyp7a1 expression of transgenic mice to be only 35% +/- 14% that in wildtype mice. |
| molecularNote | The transgene comprises one to two copies of bacterial artificial chromosome (BAC) clone RP23-291P1, whose 182-kb, C57BL/6J-derived genomic DNA insert contains the Abc11 gene, 33 kb of 5' flanking and 44 kb of 3' flanking DNA, and the entire glucose-6-phosphatase, catalytic, 2 (G6pc2) gene. Northern blot analysis of total liver RNA and western blot analysis of canalicular liver plasma membrane extracts showed about two-fold elevation of Abc11 transcript and protein levels in transgenic mice fed a chow diet; transcript levels were unaffected by consumption of a lithogenic diet for 8 weeks. |
