| First Author | Brault JB | Year | 2022 |
| Journal | EMBO Rep | Volume | 23 |
| Issue | 10 | Pages | e54605 |
| PubMed ID | 35979738 | Mgi Jnum | J:330973 |
| Mgi Id | MGI:7384186 | Doi | 10.15252/embr.202254605 |
| Citation | Brault JB, et al. (2022) RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination. EMBO Rep 23(10):e54605 |
| abstractText | Radial glial (RG) cells are the neural stem cells of the developing neocortex. Apical RG (aRG) cells can delaminate to generate basal RG (bRG) cells, a cell type associated with human brain expansion. Here, we report that aRG delamination is regulated by the post-Golgi secretory pathway. Using in situ subcellular live imaging, we show that post-Golgi transport of RAB6+ vesicles occurs toward the minus ends of microtubules and depends on dynein. We demonstrate that the apical determinant Crumbs3 (CRB3) is also transported by dynein. Double knockout of RAB6A/A' and RAB6B impairs apical localization of CRB3 and induces a retraction of aRG cell apical process, leading to delamination and ectopic division. These defects are phenocopied by knockout of the dynein activator LIS1. Overall, our results identify a RAB6-dynein-LIS1 complex for Golgi to apical surface transport in aRG cells, and highlights the role of this pathway in the maintenance of neuroepithelial integrity. |
