| First Author | Yang J | Year | 2022 |
| Journal | EBioMedicine | Volume | 78 |
| Pages | 103958 | PubMed ID | 35316682 |
| Mgi Jnum | J:322171 | Mgi Id | MGI:7257121 |
| Doi | 10.1016/j.ebiom.2022.103958 | Citation | Yang J, et al. (2022) Dynamic profiling of immune microenvironment during pancreatic cancer development suggests early intervention and combination strategy of immunotherapy. EBioMedicine 78:103958 |
| abstractText | BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) has little response to immune checkpoint inhibitors. An in-depth understanding of the immune microenvironment from a comprehensive and dynamic perspective is critical to generate effective therapeutic strategies for PDAC. METHODS: Using mass cytometry and immunohistochemistry, we explored the dynamic changes of tumor-infiltrating immune cells during the development of PDAC in a genetically engineered mouse model (Kras(G12D/+); Trp53(R172H/+); Pdx1-cre) and human specimens. PD-L1(-/-) mice were crossed with Kras(G12D/+); TgfbetaR2(flox/flox); Ptf1a-cre mice to achieve early depletion of PD-L1 in pancreatic cancer. Combination therapy of Arginase-1 (Arg-1) inhibitor and anti-PD-1 mAb was validated in syngeneic mouse models. FINDINGS: Two different stages of immunosuppression with unique features were observed in both mouse model and human specimens. Early stage of immunosuppression featured highly abundant Tregs during acinar-to-ductal metaplasia, despite of a prominent and continuous presence of effector lymphocytes. The differentiation/activation branch of Ly-6C(+) monocytes changed from a BST2(+)/MHC-II(+) phenotype to an Arg-1(+) phenotype over time during PDAC development. The late stage of immunosuppression thus featured the presence of a large number of myeloid suppressive cells together with a significant reduction of effector lymphocytes. Removal of PD-L1 from the beginning efficiently triggered anti-tumor immunity and significantly prolonged survival in PDAC-developing mice. Targeting Arg1(+) macrophages with an Arg-1 inhibitor synergized with anti-PD-1 immunotherapy and led to PDAC-specific immune memory. INTERPRETATION: By demonstrating the coevolution of histopathology and immunology in PDAC, this study highlights the necessity and value of early intervention and combinational approach in leveraging immunotherapy to treat pancreatic cancer. FUNDING: A full list of funding bodies that contributed to this study can be found in the Acknowledgements section. |
