| First Author | Boes M | Year | 2003 |
| Journal | J Immunol | Volume | 171 |
| Issue | 8 | Pages | 4081-8 |
| PubMed ID | 14530329 | Mgi Jnum | J:120180 |
| Mgi Id | MGI:3703984 | Doi | 10.4049/jimmunol.171.8.4081 |
| Citation | Boes M, et al. (2003) T cells induce extended class II MHC compartments in dendritic cells in a Toll-like receptor-dependent manner. J Immunol 171(8):4081-8 |
| abstractText | Interaction of Ag-loaded dendritic cells with Ag-specific CD4 T cells induces the formation of long tubular class II MHC-positive compartments that polarize toward the T cell. We show involvement of a Toll-like receptor-mediated signal in this unusual form of intracellular class II MHC trafficking. First, wild-type dendritic cells loaded with LPS-free Ag failed to show formation of class II-positive tubules upon Ag-specific T cell engagement, but did so upon supplementation of the Ag with low concentrations of LPS. Second, Ag-loaded myeloid differentiation factor 88 -deficient dendritic cells failed to form these tubules upon interaction with T cells, regardless of the presence of LPS. Finally, inclusion of a cell-permeable peptide that blocks TNFR-associated factor 6 function, downstream of myeloid differentiation factor 88, blocked T cell-dependent tubulation. A Toll-like receptor-dependent signal is thus required to allow Ag-loaded dendritic cells to respond to T cell contact by formation of extended endosomal compartments. This activation does not result in massive translocation of class II MHC molecules to the cell surface. |
