| First Author | Atibalentja DF | Year | 2011 |
| Journal | J Immunol | Volume | 186 |
| Issue | 3 | Pages | 1421-31 |
| PubMed ID | 21178002 | Mgi Jnum | J:168924 |
| Mgi Id | MGI:4939307 | Doi | 10.4049/jimmunol.1002587 |
| Citation | Atibalentja DF, et al. (2011) Functional redundancy between thymic CD8alpha+ and Sirp alpha+ conventional dendritic cells in presentation of blood-derived lysozyme by MHC class II proteins. J Immunol 186(3):1421-31 |
| abstractText | We evaluated the presentation of blood-derived protein Ags by APCs in the thymus. Two conventional dendritic cells (cDCs), the CD8alpha(+)Sirpalpha(-)CD11c(hi) (CD8alpha(+) cDC) and the CD8alpha(-)Sirpalpha(+)CD11c(hi) (Sirpalpha(+) cDC), were previously identified as presenting MHC class II bound peptides from hen egg white lysozyme (HEL) injected intravenously. All thymic APCs acquired the injected HEL, with the plasmacytoid dendritic cell being the best, followed by the Sirpalpha(+) cDC and the CD8alpha(+) cDC. Both cDCs induced to similar extent negative selection and regulatory T cells in HEL TCR transgenic mice, indicating a redundant role of the two cDC subsets in the presentation of blood-borne HEL. Immature dendritic cells or plasmacytoid dendritic cells were considerably less efficient. Batf3(-/-) mice, with significantly reduced numbers of CD8alpha(+) cDCs, were not impaired in HEL presentation by I-A(k) molecules of thymic APCs. Lastly, clodronate liposome treatment of TCR transgenic mice depleted blood APCs including Sirpalpha(+) cDCs without affecting the number of thymic APCs. In such treated mice, there was no effect on negative selection or regulatory T cells in mice when administering HEL, indicating that the T cell responses were mediated primarily by the cDCs localized in the thymus. |
