Primary Identifier | MGI:2385954 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Tcrd-V1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 2 was replaced with a floxed PGK-neo cassette via homologous recombination. Prior to blastocyst injection, the PGK-neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Southern blotting confirmed correct targeting of construct. |