| First Author | Richert E | Year | 2020 |
| Journal | Adv Med Sci | Volume | 65 |
| Issue | 1 | Pages | 71-77 |
| PubMed ID | 31918066 | Mgi Jnum | J:301886 |
| Mgi Id | MGI:6507283 | Doi | 10.1016/j.advms.2019.11.003 |
| Citation | Richert E, et al. (2020) CRB1(rd8) mutation influences the age-related macular degeneration phenotype of NRF2 knockout mice and favors choroidal neovascularization. Adv Med Sci 65(1):71-77 |
| abstractText | PURPOSE: We examined the influence of retinal degeneration 8 (rd8) mutation of crumbs homolog 1 (CRB1) gene on age-related macular degeneration (AMD) phenotype in nuclear factor E2-related factor 2 knock out (NRF2(-/-)) mouse model. METHODS: CRB1(rd8) mutation genotype was determined by polymerase chain reaction from tail clips in 73 NRF2(-/-) mice originating from C57BL/6J background on mixed C57BL/6J and C57BL/6N ancestry. The clinical grade of AMD-like fundus alterations was determined by funduscopy, optical coherence tomography (OCT) and fluorescein angiography (FLA) at the age of 9 or 12 months. RESULTS: Twelve NRF2(-/-) mice were wildtype CRB1(+/+), 61 NRF2(-/-) were homozygous CRB1(rd8/rd8). NRF2(-/-)CRB1(rd8/rd8) mice had a significantly higher probability to show an advanced grade (grade 4 and 5) of AMD-like fundus alterations known to appear in NRF2(-/-) mice. Choroidal neovascularization (CNV) was only detected in NRF2(-/-)CRB1(rd8/rd8) homozygous mice. CONCLUSIONS: Homozygous CRB1(rd8/rd8) mutation is common in commercial vendor mice strains of C57BL/6J origin if partly on C57BL/6N ancestry. The mutation has an influence on the extent of AMD-like retinal alterations in NRF2(-/-) mice and favors CNV formation. |
