| First Author | de Winde CM | Year | 2018 |
| Journal | J Cell Sci | Volume | 131 |
| Issue | 19 | PubMed ID | 30185523 |
| Mgi Jnum | J:266643 | Mgi Id | MGI:6201508 |
| Doi | 10.1242/jcs.214551 | Citation | de Winde CM, et al. (2018) C-type lectin-like receptor 2 (CLEC-2)-dependent dendritic cell migration is controlled by tetraspanin CD37. J Cell Sci 131(19):jcs214551 |
| abstractText | Cell migration is central to evoking a potent immune response. Dendritic cell (DC) migration to lymph nodes is dependent on the interaction of C-type lectin-like receptor 2 (CLEC-2; encoded by the gene Clec1b), expressed by DCs, with podoplanin, expressed by lymph node stromal cells, although the underlying molecular mechanisms remain elusive. Here, we show that CLEC-2-dependent DC migration is controlled by tetraspanin CD37, a membrane-organizing protein. We identified a specific interaction between CLEC-2 and CD37, and myeloid cells lacking CD37 (Cd37 (-/-)) expressed reduced surface CLEC-2. CLEC-2-expressing Cd37 (-/-) DCs showed impaired adhesion, migration velocity and displacement on lymph node stromal cells. Moreover, Cd37 (-/-) DCs failed to form actin protrusions in a 3D collagen matrix upon podoplanin-induced CLEC-2 stimulation, phenocopying CLEC-2-deficient DCs. Microcontact printing experiments revealed that CD37 is required for CLEC-2 recruitment in the membrane to its ligand podoplanin. Finally, Cd37 (-/-) DCs failed to inhibit actomyosin contractility in lymph node stromal cells, thus phenocopying CLEC-2-deficient DCs. This study demonstrates that tetraspanin CD37 controls CLEC-2 membrane organization and provides new molecular insights into the mechanisms underlying CLEC-2-dependent DC migration.This article has an associated First Person interview with the first author of the paper. |
