| First Author | Iyoda T | Year | 2010 |
| Journal | Int Immunol | Volume | 22 |
| Issue | 11 | Pages | 905-13 |
| PubMed ID | 21118907 | Mgi Jnum | J:167224 |
| Mgi Id | MGI:4867580 | Doi | 10.1093/intimm/dxq444 |
| Citation | Iyoda T, et al. (2010) Invariant NKT cell anergy is induced by a strong TCR-mediated signal plus co-stimulation. Int Immunol 22(11):905-13 |
| abstractText | Valpha14 TCR expressing invariant NK T (iNKT) cells recognize alpha-galactosylceramide (alphaGC)/CD1d complex and produce large amounts of various cytokines before the onset of the adaptive immunity. After stimulation with a high dose (2-5 mug) of alphaGC in vivo, iNKT cells in the spleen and liver become anergic in terms of the proliferation and cytokine production to subsequent stimulation. In this study, we monitor how iNKT anergy is induced. Anergized iNKT cells dramatically reduced the expression of IL-2Ralpha, and exogenous IL-2 restored the ability to proliferate and produce IL-4 but not to produce IFN-gamma. Anergized iNKT cells expressed high levels of programmed death-1 (PD-1). However, iNKT cells in PD-1-deficient mice became anergic as a result of alphaGC injection, as do normal mice. Furthermore, anti-PD-1 blocking mAb was unable to restore their responsiveness. When iNKT cells were stimulated with immobilized anti-CD3 in the presence or absence of anti-CD28, they produced cytokines in a dose-dependent manner. Unlike in naive CD4 T cells, the strong TCR-mediated signaling with co-stimulation renders them anergic to any subsequent stimulation with alphaGC and spleen dendritic cells (DCs). Moreover, iNKT cells also became anergic after stimulation with phorbol-12-myristate-13-acetate + ionophore. Finally, the injection of alphaGC-pulsed DCs was more potent in inducing anergy than B cells. These results indicate that strong TCR-mediated activation with co-stimulation provides signals that induce the anergic state in iNKT cells. |
