| First Author | Lin Y | Year | 2016 |
| Journal | Ann Clin Lab Sci | Volume | 46 |
| Issue | 1 | Pages | 32-43 |
| PubMed ID | 26927340 | Mgi Jnum | J:333627 |
| Mgi Id | MGI:6757337 | Citation | Lin Y, et al. (2016) The Protective Effect of HIF-1alpha in T Lymphocytes on Cardiac Damage in Diabetic Mice. Ann Clin Lab Sci 46(1):32-43 |
| abstractText | Diabetic cardiomyopathy (DCM) is associated with functional and structural pathological changes such as hypoxia and inflammation. Hypoxia-inducible factor-1alpha(HIF-1alpha) is a core transcription factor for restoring homeostasis in intracellular oxygen and has a crucial role in preventing the development of DCM. However, the effect of HIF-1alpha in T lymphocytes on DCM has not been reported. We established T lymphocyte-specific HIF-1alpha knockout homozygous mice that were injected with streptozotocin (STZ) for establishing diabetic models. Random blood glucose (RBG), body weight and the survival rate were detected. The cardiac pathological changes were evaluated by periodic acid-Schiff (PAS) staining, hematoxylin-eosin staining (HE) and Masson collagen staining. Cardiac function measurements were obtained by echocardiography. We observed the infiltration of T lymphocytes/CD3+ in the hearts by immunofluorescence stain. Also, we isolated splenic lymphocytes which would be in vitro cultured in the environment of high glucose and hypoxia. HIF-1alpha protein level of splenic lymphocytes was measured by Western blot. The results of this study indicate that the expression of HIF-1alpha in lymphocytes is activated in the complex environment of DCM consisting of hypoxia and high glucose. Also, HIF-1alpha in T cells plays a critical role in avoiding damage to the diabetic cardiac tissues. |
