| First Author | Nakamura-Ishizu A | Year | 2012 |
| Journal | Dev Biol | Volume | 363 |
| Issue | 1 | Pages | 106-14 |
| PubMed ID | 22226979 | Mgi Jnum | J:182708 |
| Mgi Id | MGI:5316353 | Doi | 10.1016/j.ydbio.2011.12.027 |
| Citation | Nakamura-Ishizu A, et al. (2012) The formation of an angiogenic astrocyte template is regulated by the neuroretina in a HIF-1-dependent manner. Dev Biol 363(1):106-14 |
| abstractText | The vascular and nervous systems display a high degree of cross-talk and depend on each other functionally. In the vascularization of the central nervous system, astrocytes have been thought to sense tissue oxygen levels in hypoxia-inducible factors (HIFs)-dependent manner and control the vascular growth into the hypoxic area by secreting VEGF. However, recent genetic evidences demonstrate that not only astrocyte HIFs but also astrocyte VEGF expression is dispensable for developmental angiogenesis of the retina. This study demonstrates that hypoxia-inducible factor 1 alpha subunit (HIF-1alpha), a key transcription factor involved in cellular responses to hypoxia, is most abundantly expressed in the neuroretina, especially retinal progenitor cells (RPCs). A neuroretina-specific knockout of HIF-1alpha (alphaCre(+)Hif1alpha(flox/flox)) showed impaired vascular development characterized by decreased tip cell filopodia and reduced vessel branching. The astrocyte network was hypoplastic in alphaCre(+)Hif1alpha(flox/flox) mice. Mechanistically, platelet-derived growth factor A (PDGF-A), a mitogen for astrocytes, was downregulated in the neuroretina of alphaCre(+)Hif1alpha(flox/flox) mice. Supplementing PDGF-A restored reduced astrocytic and vascular density in alphaCre(+)Hif1alpha(flox/flox) mice. Our data demonstrates that the neuroretina but not astrocytes acts as a primary oxygen sensor which ultimately controls the retinal vascular development by regulating an angiogenic astrocyte template. |
