| First Author | Kumar V | Year | 2022 |
| Journal | Front Cardiovasc Med | Volume | 9 |
| Pages | 992653 | PubMed ID | 36093172 |
| Mgi Jnum | J:329016 | Mgi Id | MGI:7339684 |
| Doi | 10.3389/fcvm.2022.992653 | Citation | Kumar V, et al. (2022) CD4(+) T-lymphocytes exhibit biphasic kinetics post-myocardial infarction. Front Cardiovasc Med 9:992653 |
| abstractText | CD4(+) T-cells facilitate wound healing post-myocardial infarction (MI) but promote left-ventricular (LV) remodeling during ischemic heart failure (HF; 8 weeks post-MI). Therefore, it is critical to understand if sustained CD4(+) T-cell activation leads to this pathological response, or if phenotypically different T-cells are activated during MI vs. HF. Using flow cytometry, we found that cardiac CD4(+) T-cells exhibit two distinct patterns of transmigration. First pattern consisted of a rapid CD4(+) T-cell response with maximal levels seen at 3 days post-MI which return to baseline by 14 days. However, during HF we observed a 2nd phase of activation and CD4(+) T-cells were approximately 20-fold higher in HF as compared to sham-operated mice. Importantly, these biphasic kinetics were observed with all major T-cell subsets such as Th1, Th2, Th17, and regulatory T-cells suggesting a global change. To determine the role of this 2nd peak of T-cell activation, CD4-iDTR mice were generated and treated with DT every 10 from 28 days post-MI to deplete CD4(+) T-cells during chronic HF. While littermate control mice showed increased end-systolic and end-diastolic volumes (ESV and EDV) and decreased ejection fraction (EF) from 4 to 8 weeks post-MI, depletion of CD4(+) T-cells in Cre + mice significantly blunted LV remodeling and inhibited progressive increases in the EDV and ESV, and reduction in EF. This suggests that CD4(+) T-cell responses occurring during HF are different than those occurring during MI and promote LV remodeling and progressive cardiac dysfunction. Temporal immunomodulation of CD4(+) T-cells could be a translatable modality for ischemic HF. |
