| First Author | Tanaka S | Year | 2014 |
| Journal | J Immunol | Volume | 193 |
| Issue | 12 | Pages | 6152-60 |
| PubMed ID | 25398328 | Mgi Jnum | J:324537 |
| Mgi Id | MGI:6859072 | Doi | 10.4049/jimmunol.1303422 |
| Citation | Tanaka S, et al. (2014) CCAAT/enhancer-binding protein alpha negatively regulates IFN-gamma expression in T cells. J Immunol 193(12):6152-60 |
| abstractText | Humoral immunity, including Ab switching and somatic hypermutation, is critically regulated by CD4(+) T cells. T follicular helper (Tfh) cells have been recently shown to be a distinct T cell subset important in germinal center reactions. The transcriptional regulation of Tfh cell development and function has not been well understood. In this study, we report that C/EBPalpha, a basic region/leucine zipper transcription factor, is highly expressed in Tfh cells. Cebpa-deficient CD4(+) T cells exhibit enhanced IFN-gamma expression in vitro and in vivo. T cell-specific Cebpa knockout mice, although not defective in Tfh cell generation, produce significantly increased levels of IgG2a/b and IgG3 following immunization with a protein Ag. Moreover, C/EBPalpha binds to the Ifng gene and inhibits T-bet-driven Ifng transcription in a DNA binding-dependent manner. Our study thus demonstrates that C/EBPalpha restricts IFN-gamma expression in T cells to allow proper class switching by B cells. |
