| First Author | Takada K | Year | 2011 |
| Journal | J Immunol | Volume | 186 |
| Issue | 2 | Pages | 775-83 |
| PubMed ID | 21160050 | Mgi Jnum | J:168765 |
| Mgi Id | MGI:4938211 | Doi | 10.4049/jimmunol.1000094 |
| Citation | Takada K, et al. (2011) Kruppel-like factor 2 is required for trafficking but not quiescence in postactivated T cells. J Immunol 186(2):775-83 |
| abstractText | The transcription factor Kruppel-like factor 2 (KLF2) was proposed to regulate genes involved in cell cycle entry and T cell trafficking; however, the physiological role of its expression in postactivated T cells is not well defined. Previous studies suggested that the cytokines IL-2 and IL-15 differentially regulate KLF2 re-expression in postactivation T cells and that these cytokines also influence effector versus memory T cell differentiation. Using conditional and inducible KLF2-knockout model systems, we tested the specific role of KLF2 expression in activated CD8(+) T cells cultured with these cytokines. KLF2 was required for effective transcription of sphingosine-1-phosphate receptor-1 (S1P(1)) and CD62L in postactivation T cells. However, although different cytokines dramatically altered the expression of cell-cycle-related genes, endogenous KLF2 had a minimal impact. Correspondingly, KLF2-deficient T cells showed dysregulated trafficking but not altered proliferative characteristics following in vivo responses to Ag. Thus, our data help to define KLF2-dependent and -independent aspects of activated CD8(+) T cell differentiation and argue against a physiological role in cell cycle regulation. |
