| First Author | Seibler J | Year | 2003 |
| Journal | Nucleic Acids Res | Volume | 31 |
| Issue | 4 | Pages | e12 |
| PubMed ID | 12582257 | Mgi Jnum | J:126518 |
| Mgi Id | MGI:3761453 | Doi | 10.1093/nar/gng012 |
| Citation | Seibler J, et al. (2003) Rapid generation of inducible mouse mutants. Nucleic Acids Res 31(4):e12 |
| abstractText | We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol. |
