| Primary Identifier | MGI:2386550 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Nes-cre)2Wme |
| Strain of Origin | (C57BL/6 x CBA)F2 | Is Recombinase | true |
| Is Wild Type | false |
| description | Mice carrying this "balancer 2" transgene show greater variability and instability in their generation of mosaics than do transgenic mice carrying the "balancer 1" transgene. |
| molecularNote | The transgenic construct contained a nestin promoter followed by cre recombinase sequence adjoined to a nuclear localization signal. Intron 2 of the nestin gene was included downstream of a polyadenylation signal. While the endogenous nestin promoter is CNS-specific, Southern blot analysis showed the ubiquitous expression of cre in transgenic mice. Variable mosaicism/partial deletion of the loxP-flanked allele occurs, and it may be detected in all adult organs including germ line cells. |
