| First Author | Zhao Y | Year | 2022 |
| Journal | Nat Commun | Volume | 13 |
| Issue | 1 | Pages | 3439 |
| PubMed ID | 35715406 | Mgi Jnum | J:326743 |
| Mgi Id | MGI:7311032 | Doi | 10.1038/s41467-022-31050-8 |
| Citation | Zhao Y, et al. (2022) 3D structure and in situ arrangements of CatSper channel in the sperm flagellum. Nat Commun 13(1):3439 |
| abstractText | The sperm calcium channel CatSper plays a central role in successful fertilization as a primary Ca(2+) gateway. Here, we applied cryo-electron tomography to visualize the higher-order organization of the native CatSper complex in intact mammalian sperm. The repeating CatSper units form long zigzag-rows along mouse and human sperm flagella. Above each tetrameric channel pore, most of the extracellular domains form a canopy that interconnects to a zigzag-shaped roof. Murine CatSper contains an additional wing-structure connected to the tetrameric channel. The intracellular domains link two neighboring channels to a diagonal array, suggesting a dimer formation. Fitting of an atomic model of isolated monomeric CatSper to the in situ map reveals supramolecular interactions and assembly of the CatSper complex. Loss of EFCAB9-CATSPERzeta alters the architecture and interactions of the channels, resulting in fragmentation and misalignment of the zigzag-rows and disruption of flagellar movement in Efcab9(-/-) sperm. This work offers unique insights into the structural basis for understanding CatSper regulation of sperm motility. |
