| First Author | Martinez-Sanchez A | Year | 2018 |
| Journal | FASEB J | Volume | 32 |
| Issue | 5 | Pages | 2587-2600 |
| PubMed ID | 29269398 | Mgi Jnum | J:273812 |
| Mgi Id | MGI:6294509 | Doi | 10.1096/fj.201701100R |
| Citation | Martinez-Sanchez A, et al. (2018) MiR-184 expression is regulated by AMPK in pancreatic islets. FASEB J 32(5):2587-2600 |
| abstractText | AMPK is a critical energy sensor and target for widely used antidiabetic drugs. In beta cells, elevated glucose concentrations lower AMPK activity, and the ablation of both catalytic subunits [beta-cell-specific AMPK double-knockout (betaAMPKdKO) mice] impairs insulin secretion in vivo and beta-cell identity. MicroRNAs (miRNAs) are small RNAs that silence gene expression that are essential for pancreatic beta-cell function and identity and altered in diabetes. Here, we have explored the miRNAs acting downstream of AMPK in mouse and human beta cells. We identified 14 down-regulated and 9 up-regulated miRNAs in betaAMPKdKO vs. control islets. Gene ontology analysis of targeted transcripts revealed enrichment in pathways important for beta-cell function and identity. The most down-regulated miRNA was miR-184 (miR-184-3p), an important regulator of beta-cell function and compensatory expansion that is controlled by glucose and reduced in diabetes. We demonstrate that AMPK is a potent regulator and an important mediator of the negative effects of glucose on miR-184 expression. Additionally, we reveal sexual dimorphism in miR-184 expression in mouse and human islets. Collectively, these data demonstrate that glucose-mediated changes in AMPK activity are central for the regulation of miR-184 and other miRNAs in islets and provide a link between energy status and gene expression in beta cells.-Martinez-Sanchez, A., Nguyen-Tu, M.-S., Cebola, I., Yavari, A., Marchetti, P., Piemonti, L., de Koning, E., Shapiro, A. M. J., Johnson, P., Sakamoto, K., Smith, D. M., Leclerc, I., Ashrafian, H., Ferrer, J., Rutter, G. A. MiR-184 expression is regulated by AMPK in pancreatic islets. |
