| First Author | Xia L | Year | 2012 |
| Journal | Neurosci Lett | Volume | 521 |
| Issue | 1 | Pages | 20-5 |
| PubMed ID | 22622174 | Mgi Jnum | J:188810 |
| Mgi Id | MGI:5442255 | Doi | 10.1016/j.neulet.2012.05.046 |
| Citation | Xia L, et al. (2012) Ventral hippocampal molecular pathways and impaired neurogenesis associated with 5-HT(1)A and 5-HT(1)B receptors disruption in mice. Neurosci Lett 521(1):20-5 |
| abstractText | The serotonergic system has been widely implicated in stress related psychiatric disorders such as depression and anxiety. Generation of receptor knockout mice has offered a new approach to study processes underlying anxiety. For instance, knockout mice for both 5-HT(1A) and 5-HT(1B) receptors (5-HT(1A/1B)(-/-)) display an anxious phenotype, associated with robust physiological and neurochemical changes related to brain serotonin function. As ventral hippocampus is a key region in the mediation and genesis of anxiety, we explored the transcriptome changes induced by the genetic inactivation of these two receptors in 5-HT(1A/1B)(-/-) mice. Dissociation of ventral vs. dorsal hippocampus was confirmed by the over-expression of selective markers in both regions. 723 genes were observed up/down regulated in 5-HT(1A/1B)(-/-) mice. Using Ingenuity, biological networks and signal transduction pathway analysis corresponding to the identified gene revealed putative dysregulation of nervous system development and function, especially genes associated with long-term potentiation and adult neurogenesis (including Bdnf, Camk2a, Camk4, and Klf9). Furthermore, immunohistochemistry experiments studying adult hippocampal neurogenesis in adult 5-HT(1A/1B)(-/-) mice showed a decreased survival, but not proliferation of newborn cells in our model. |
