| First Author | El-Gaby M | Year | 2016 |
| Journal | Cell Rep | Volume | 16 |
| Issue | 8 | Pages | 2259-2268 |
| PubMed ID | 27524609 | Mgi Jnum | J:238949 |
| Mgi Id | MGI:5824617 | Doi | 10.1016/j.celrep.2016.07.057 |
| Citation | El-Gaby M, et al. (2016) Archaerhodopsin Selectively and Reversibly Silences Synaptic Transmission through Altered pH. Cell Rep 16(8):2259-68 |
| abstractText | Tools that allow acute and selective silencing of synaptic transmission in vivo would be invaluable for understanding the synaptic basis of specific behaviors. Here, we show that presynaptic expression of the proton pump archaerhodopsin enables robust, selective, and reversible optogenetic synaptic silencing with rapid onset and offset. Two-photon fluorescence imaging revealed that this effect is accompanied by a transient increase in pH restricted to archaerhodopsin-expressing boutons. Crucially, clamping intracellular pH abolished synaptic silencing without affecting the archaerhodopsin-mediated hyperpolarizing current, indicating that changes in pH mediate the synaptic silencing effect. To verify the utility of this technique, we used trial-limited, archaerhodopsin-mediated silencing to uncover a requirement for CA3-CA1 synapses whose afferents originate from the left CA3, but not those from the right CA3, for performance on a long-term memory task. These results highlight optogenetic, pH-mediated silencing of synaptic transmission as a spatiotemporally selective approach to dissecting synaptic function in behaving animals. |
