| Primary Identifier | MGI:2387445 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Ret |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cDNA fragment encoding the intracelluar portion of the human RET9 isoform was fused in-frame with mouse exon 11 immediately downstream of the transmembrane domain-coding region. A floxed PGK-neo cassette followed the human cDNA sequence for positive selection. RT-PCR analysis and Western blot using allele-specific primers and antibodies, respectively, confirmed expression of the chimeric gene product in homozygous mutant animals. Semiquanititative RT-PCR and Western blot also determined the expression level of the mutant allele was similar to that of wild-type. |
