| First Author | Pospichalova V | Year | 2011 |
| Journal | Genesis | Volume | 49 |
| Issue | 3 | Pages | 142-51 |
| PubMed ID | 21309068 | Mgi Jnum | J:170337 |
| Mgi Id | MGI:4946336 | Doi | 10.1002/dvg.20719 |
| Citation | Pospichalova V, et al. (2011) Generation of two modified mouse alleles of the Hic1 tumor suppressor gene. Genesis 49(3):142-51 |
| abstractText | HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine 'reporter' mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence. genesis 49:142-151, 2011. (c) 2011 Wiley-Liss, Inc. |
