| First Author | Garcia-Santos D | Year | 2014 |
| Journal | Blood | Volume | 123 |
| Issue | 14 | Pages | 2269-77 |
| PubMed ID | 24511086 | Mgi Jnum | J:210164 |
| Mgi Id | MGI:5569669 | Doi | 10.1182/blood-2013-04-496760 |
| Citation | Garcia-Santos D, et al. (2014) Heme oxygenase 1 is expressed in murine erythroid cells where it controls the level of regulatory heme. Blood 123(14):2269-77 |
| abstractText | Heme is essential for the function of all aerobic cells. However, it can be toxic when it occurs in a non-protein-bound form; cells maintain a fine balance between heme synthesis and catabolism. The only physiological mechanism of heme degradation is by heme oxygenases (HOs). The heme-inducible isoform, HO-1, has been extensively studied in numerous nonerythroid cells, but virtually nothing is known about the expression and potential significance of HO-1 in developing red blood cells. We have demonstrated that HO-1 is present in erythroid cells and that its expression is upregulated during erythroid differentiation. Overexpression of HO-1 in erythroid cells impairs hemoglobin synthesis, whereas HO-1 absence enhances hemoglobinization in cultured erythroid cells. Based on these results, we conclude that HO-1 controls the regulatory heme pool at appropriate levels for any given stage of erythroid differentiation. In summary, our study brings to light the importance of HO-1 expression for erythroid development and expands our knowledge about the fine regulation of hemoglobin synthesis in erythroid cells. Our results indicate that HO-1 plays an important role as a coregulator of the erythroid differentiation process. Moreover, HO-1 expression must be tightly regulated during red blood cell development. |
