| Primary Identifier | MGI:2388355 | Allele Type | Targeted |
| Gene | Cbfb | Transmission | Germline |
| Strain of Origin | 129S6/SvEvTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | MYH11 sequence isolated from a human CBFB-MYH11 cDNA, resulting from a chromosome 16 inversion common in human acute myeloid leukemia subtype M4Eo, was inserted into a StuI site in exon 5 of the endogenous gene by homologous recombination. The targeting vector also contained a neo cassette inserted into intron 5 for selection. Northern and Western blot analyses confirmed the production of fusion transcript and protein in heterozygous mutant embryos. |
