| First Author | Soh S | Year | 2023 |
| Journal | Cell Commun Signal | Volume | 21 |
| Issue | 1 | Pages | 158 |
| PubMed ID | 37370133 | Mgi Jnum | J:337431 |
| Mgi Id | MGI:7494575 | Doi | 10.1186/s12964-023-01143-y |
| Citation | Soh S, et al. (2023) Adiponectin affects the migration ability of bone marrow-derived mesenchymal stem cells via the regulation of hypoxia inducible factor 1alpha. Cell Commun Signal 21(1):158 |
| abstractText | BACKGROUND: Bone marrow (BM) is progressively filled with adipocytes during aging process. Thus, BM adipocytes-derived adiponectin (APN) affects the function of bone marrow-derived mesenchymal stem cells (BMSCs). However, little is known about the effect of APN on migration ability of BMSCs cultured under hypoxic conditions, which is similar to the BM microenvironment. RESULTS: We found that the population and migration ability of BMSCs from APN KO mice was higher than that of WT mice due to increased stability of hypoxia inducible factor 1alpha (HIF1alpha). Stem cell factor (SCF)-activated STAT3 stimulated the induction of HIF1alpha which further stimulated SCF production, indicating that the SCF/STAT3/HIF1alpha positive loop was highly activated in the absence of APN. It implies that APN negatively regulated this positive loop by stimulating HIF1alpha degradation via the inactivation of GSK3beta. Furthermore, APN KO BMSCs were highly migratory toward EL-4 lymphoma, and the interaction between CD44 in BMSCs and hyaluronic acid (HA) from EL-4 enhanced the migration of BMSCs. On the other hand, the migrated BMSCs recruited CD8(+) T cells into the EL-4 tumor tissue, resulting in the retardation of tumor growth. Additionally, gradually increased APN in BM on the aging process affects migration and related functions of BMSCs, thus aged APN KO mice showed more significant suppression of EL-4 growth than young APN KO mice due to higher migration and recruitment of CD8(+) T cells. CONCLUSION: APN deficiency enhances CD44-mediated migration ability of BMSCs in the hypoxic conditions by the SCF/STAT3/HIF1alpha positive loop and influences the migration ability of BMSCs for a longer time depending on the aging process. Video Abstract. |
