| First Author | Shiratori K | Year | 2019 |
| Journal | Biochem Biophys Res Commun | Volume | 511 |
| Issue | 2 | Pages | 330-335 |
| PubMed ID | 30791982 | Mgi Jnum | J:291164 |
| Mgi Id | MGI:6442733 | Doi | 10.1016/j.bbrc.2019.02.046 |
| Citation | Shiratori K, et al. (2019) Epidermal loss of phospholipase Cdelta1 attenuates irritant contact dermatitis. Biochem Biophys Res Commun 511(2):330-335 |
| abstractText | Irritant contact dermatitis (ICD) is one of the most common inflammatory skin diseases caused by exposure to chemical irritants. Since chemical irritants primarily damage keratinocytes, these cells play a pivotal role in ICD. One of the phosphoinositide-metabolizing enzymes, phospholipase C (PLC) delta1, is abundantly expressed in keratinocytes. However, the role of PLCdelta1 in ICD remains to be clarified. Here, we found that croton oil (CrO)-induced ear swelling, a feature of ICD, was attenuated in keratinocyte-specific PLCdelta1 knockout mice (PLCdelta1 cKO mice). Dendritic epidermal T cells (DETCs), which have a protective role against ICD, were activated in the epidermis of the PLCdelta1 cKO mice. In addition, the skin of CrO-treated PLCdelta1 cKO mice showed increased infiltration of Gr1(+)CD11b(+) myeloid cells. Of note, elimination of Gr1(+)CD11b(+) myeloid cells restored CrO-induced ear swelling in PLCdelta1 cKO mice to a similar level as that in control mice. Taken together, our results strongly suggest that epidermal loss of PLCdelta1 protects mice from ICD through induction of Gr1(+)CD11b(+) myeloid cells and activation of DETCs. |
